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Tris-MES-SDS Buffer

Buffer Description

Tris-MES-SDS buffer is designed for separating low-molecular-weight proteins with Bis-Tris gels at neutral pH, enabling faster protein migration than MOPS buffer; its instant granules dissolve in 1 L of deionized water to prepare 1X solution (50 mM Tris, 50 mM MES, 0.1% SDS, 1 mM EDTA, pH 6.8–7.6 at 25°C, no pH adjustment needed).

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Protein Electrophoresis Series
Pre-stained Protein Ladder
Broad Range High Range Low Range
Unstained Protein Ladder
SDS-PAGE Gel Preparation Kit
Electrophoresis Buffer
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Electroblotting Transfer Buffer
Membrane Wash Buffer
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Catalog No. Pack Size Price Quantity
X10205-010 10 x 1 L (a box) Price Inquiry
X10205-100 100 x 1 L (a box) Price Inquiry

Description

Our Tris-MES-SDS buffer is engineered for the separation of low-molecular-weight proteins via Bis-Tris polyacrylamide gel electrophoresis under neutral pH conditions. Compared with MOPS buffer systems, MES-based buffers allow proteins to migrate at a faster rate, making it ideal for experiments focused on small protein separation.

Features

  • ● Facilitates the easy and quick preparation of 1X Tris-MES-SDS aqueous buffer solution with straightforward operational steps.
  • ● Fast-dissolving formula: Simply dissolve the Tris-MES-SDS buffer instant granules in 1 liter of deionized water. Once fully dissolved, the resulting buffer contains 50 mM Tris, 50 mM MES, 0.1% SDS, and 1 mM EDTA, with a pH range of 6.8–7.6 at 25°C (no additional pH adjustment required).
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Pre-stained Protein Ladder

Enables real-time visualization of protein migration during electrophoresis — no post-run staining required.

Unstained Protein Ladder

Free from dye interference; ideal for accurate protein quantification and sensitive detection.

SDS-PAGE Gel Preparation Kit

An all-in-one solution for preparing SDS-PAGE gels, compatible with a broad range of electrophoresis units.

Electroblotting Transfer Buffer

Formulated for efficient, high-integrity protein transfer from polyacrylamide gels to membranes.

Membrane Wash Buffer

Effectively removes non-specific antibody binding, minimizing background and improving signal-to-noise ratio.

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