Pre-stained Protein Ladder [X10127E]

X10127E is a ready-to-use pre-stained protein ladder containing 11 recombinant proteins (10-180 kDa). Features: Three dyes produce vivid bands (mostly blue; 70 kDa orange-red, 25 kDa green in SDS-PAGE); No pre-use preparation (heating/dilution/reducing agents) needed; Molecular weights calibrated against commercial unstained standards. Applications: Monitors SDS-PAGE separation, verifies Western blot transfer (PVDF/nitrocellulose), estimates target protein molecular weight. Shipping & Storage: Shipped at 4–8℃ with ice packs; store at -20℃ immediately upon receipt.
Catalog No. Pack Size Price Quantity
X10127E -10 x 250 μL 10 x 250 μL Catalog price:¥3570.00
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X10127E - 2 x 250 μL 2 x 250 μL Catalog price:¥892.50
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Description

The X10127E X-biotechTM Pre-stained Protein Ladder is a ready-to-use pre-stained mixture of 11 recombinant proteins ranging from 10 kDa to 180 kDa. Three different dyes are bound to the proteins, producing a brightly colored ladder. When separated by SDS-PAGE, most proteins exhibit a blue color, except for two reference bands: 70 kDa (orange-red) and 25 kDa (green).

Important Information

  • X10127E contains 11 protein bands of 10, 15, 20, 25, 30, 40, 50, 70, 100, 130, and 180 kDa.
  • The indicated apparent molecular weights were calibrated against commercially available unstained protein standards under defined electrophoresis conditions.
  • Do not heat the product above room temperature.
ItemSpecification
Cat. No. X10127E
Product Size 2 × 250 μL / 10 × 250 μL
MW Range 10 - 180 kDa
Band Number 11
Markered Bands 25, 70 kDa
Band Color Orange-Red/Green/Blue
Storage 4°C for 2 months,
-20°C for 2 years

SDS-PAGE band profile of this Pre-stained Protein Ladder

SDS-PAGE band profile

Images are from a 4-20% Tris-glycine gel (SDS-PAGE) and subsequent transfer to membrane.

Migration patterns of this Pre-stained Protein Ladder indifferent electrophoretic conditions

Migration patterns of Protein Ladder

The apparent molecular weight of each protein (kDa) has been determined by calibration of each protein against an unstained protein ladder in specific electrophoresis conditions.

Migration patterns were determined using commercial gels.

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