Pre-stained Protein Ladder [X10119]

X10119 is a ready-to-use pre-stained protein ladder containing 9 recombinant proteins (8-250 kDa). Features: Four dyes produce vivid bands (mostly blue; 70 kDa orange-red, 27 kDa orange and 8 kDa green in SDS-PAGE); No pre-use preparation (heating/dilution/reducing agents) needed; Molecular weights calibrated against commercial unstained standards. Applications: Monitors SDS-PAGE separation, verifies Western blot transfer (PVDF/nitrocellulose), estimates target protein molecular weight. Shipping & Storage: Shipped at 4–8℃ with ice packs; store at -20℃ immediately upon receipt.
Catalog No. Pack Size Price Quantity
X10119 - 10 x 250 μL 10 x 250 μL Catalog price:¥3944.00
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X10119 - 2 x 250 μL 2 x 250 μL Catalog price:¥986.00
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Description

The X10119 X-biotechTM Pre-stained Protein Ladder is a ready-to-use pre-stained mixture of 9 recombinant proteins ranging from 8 kDa to 250 kDa. Four different dyes are bound to the proteins, producing a brightly colored ladder. When separated by SDS-PAGE, most proteins exhibit a blue color, except for three reference bands: 70 kDa (orange-red), 27 kDa (orange) and 8 kDa (green).

Important Information

  • X10119 contains 9 protein bands of 8, 16.5, 27, 33, 52, 70, 95, 130, and 250 kDa.
  • The indicated apparent molecular weights were calibrated against commercially available unstained protein standards under defined electrophoresis conditions.
  • Do not heat the product above room temperature.
ItemSpecification
Cat. No. X10119
Product Size 2 × 250 μL / 10 × 250 μL
MW Range 8 - 250 kDa
Band Number 9
Markered Bands 8, 27, 70 kDa
Band Color Orange-Red/Orange/Green/Blue
Storage 4°C for 2 months,
-20°C for 2 years

SDS-PAGE band profile of this Pre-stained Protein Ladder

SDS-PAGE band profile

Images are from a 4-20% Tris-glycine gel (SDS-PAGE) and subsequent transfer to membrane

Migration patterns of this Pre-stained Protein Ladder indifferent electrophoretic conditions

Migration patterns of Protein Ladder

The apparent molecular weight of each protein (kDa) has been determined by calibration of each protein against an unstained protein ladder in specific electrophoresis conditions.

Migration patterns were determined using commercial gels.

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